@Article{oncologie.2022.025693,
AUTHOR = {Linpei Wang, Xiaoqiu Ma, Youqi Chen, Jiahui Zhang, Jiawei Zhang, Wei Wang, Shaojian Chen},
TITLE = {MiR-145-5p Suppresses Hepatocellular Carcinoma Progression by Targeting ABHD17C},
JOURNAL = {Oncologie},
VOLUME = {24},
YEAR = {2022},
NUMBER = {4},
PAGES = {897--912},
URL = {http://www.techscience.com/oncologie/v24n4/51021},
ISSN = {1765-2839},
ABSTRACT = {<b>Background: </b>MicroRNA-145-5p (miR-145-5p) reportedly inhibits hepatocellular carcinoma (HCC) by targeting
ARF6, SPATS2, CDCA3, KLF5, and NRAS, indicating that miR-145-5p plays an important role in the occurrence
and development of HCC by regulating the expression of various genes. In this study, we aimed to explore novel
downstream targets of miR-145-5p and elucidate the potential mechanism of miR-145-5p in HCC. <b>Materials and
Methods: </b>A bioinformatics analysis was performed to determine the clinical significance of miR-145-5p and
alpha/beta hydrolase domain-containing protein 17C (ABHD17C) in patients with HCC. The ability of Hep3B
cells to proliferate, migrate, and invade was examined after overexpression of miR-145-5p and ABHD17C or
knockdown of ABHD17C. Tumorigenesis of Hep3B cells overexpressing miR-145 was detected using in vivo
experiments. <b>Results:</b> miR-145-5p was downregulated in HCC tissues, and this was associated with poor prognosis in patients with HCC. Based on the bioinformatics analysis, miR-145-5p was predicted to target ABHD17C,
as demonstrated by a luciferase reporter assay. ABHD17C downregulation inhibited cell viability, migration, and
invasion and arrested the cell cycle. Overexpression of miR-145-5p significantly reduced the expression of
ABHD17C. Moreover, ABHD17C expression was elevated in HCC tissues, which was associated with an unfavorable prognosis. Re-expressing ABHD17C into HCC cells rescued the suppressed cell viability, migration, and
invasion mediated by ectopic expression of miR-145-5p. Importantly, miR-145-5p suppressed tumor growth in
mice and downregulated the levels of Ki67 and ABHD17C in tumor. <b>Conclusion:</b> miR-145-5p could attenuate
HCC progression via suppressing ABHD17C.},
DOI = {10.32604/oncologie.2022.025693}
}