@Article{or.2026.082424,
AUTHOR = {Abtin Tondar, David Hervás Marín, Laura Calvet Liñán, Asim Kumar Bepari},
TITLE = {Concordant Shared Transcriptomic Signatures and Candidate Regulatory Features in Chronic Lymphocytic Leukemia and Multiple Myeloma},
JOURNAL = {Oncology Research},
VOLUME = {},
YEAR = {},
NUMBER = {},
PAGES = {{pages}},
URL = {http://www.techscience.com/or/online/detail/27657},
ISSN = {1555-3906},
ABSTRACT = {<b>Background:</b> Chronic lymphocytic leukemia (CLL) and multiple myeloma (MM) are B-cell malignancies with distinct cellular origins and microenvironmental dependencies. We aimed to identify concordant transcriptomic signatures and candidate transcriptional regulatory features between CLL CD19-positive B cells and MM-associated bone marrow-derived mesenchymal stromal cells (MSCs). <b>Methods:</b> Public Gene Expression Omnibus bulk RNA sequencing datasets were analyzed separately within each context using DESeq2. Differentially expressed genes (DEGs) were defined using adjusted <i>p</i>-value &lt; 0.05 and absolute log<sub>2</sub> fold change &gt; 1. Cross-disease analyses assessed overlap, directionality, log<sub>2</sub> fold-change concordance, expressed-gene background-adjusted enrichment, coexpression structure, and transcription factor annotation. <b>Results:</b> We found a focused concordant gene-level signature shared across contexts. We identified 5965 DEGs in CLL and 1021 DEGs in MM; 323 were shared, and 262 were concordantly regulated, including 52 upregulated and 210 downregulated genes in both contexts. These genes showed strong log<sub>2</sub> fold change concordance between CLL and MM. No Gene Ontology or Kyoto Encyclopedia of Genes and Genomes terms remained significant after expressed-gene background correction, supporting stronger gene-level than pathway-level evidence. Exploratory coexpression analysis identified PSMA3-AS1, SNORD58A, 100124516, MSS51, and 652966 as the top degree-ranked hubs and seven shared differentially expressed transcription factor candidates: MAFB, MYB, CCDC17, MYSM1, ZMAT1, ZNF491, and ZNF789. Expression-matched permutation analysis did not support global transcription factor enrichment. <b>Conclusion:</b> These findings support a cross-contextual concordant transcriptomic signature shared by CLL CD19-positive B cells and MM-associated MSCs, warranting validation in harmonized cohorts and experiments.},
DOI = {10.32604/or.2026.082424}
}