TY  - EJOU
AU  - Qin, Yun 
AU  - Tang, Xicai 
AU  - Liu, Mingxing 

TI  - Tumor-Suppressor Gene NBPF1 Inhibits Invasion and PI3K/mTOR Signaling in Cervical Cancer Cells
T2  - Oncology Research

PY  - 2015
VL  - 23
IS  - 1-2
SN  - 1555-3906

AB  - The purpose of this study was to assess the effects of NBPF1 expression on cervical cancer cell invasion and 
apoptosis and to illustrate its potential mechanism. Human cervical cancer HeLa cells were transfected with 
the constructed siNBPF1 or pcDNA3.1-NBPF1 vectors. Effects of NBPF1 expression on cell invasion ability and cell apoptosis were analyzed using the Matrigel method and an Annexin V-FITC cell apoptosis kit, 
respectively. In addition, cell apoptosis-related proteins involved with the PI3K/mTOR signaling pathway were 
analyzed using Western blot. Remediation experiments were conducted to verify the effects of NBPF1 expression on cell invasion and apoptosis. Compared to the control, mRNA and protein expressions of NBPF1 were 
significantly decreased when cells were transfected with siNBPF1 (<i>p</i><0.05), which was contrary to the results 
of cells transfected with pcDNA3.1-NBPF1. Overexpression of NBPF1 significantly suppressed HeLa cell 
invasion but promoted cell apoptosis (<i>p</i><0.05). Overexpression of NBPF1 performed a significant inhibitory 
role on PI3K/mTOR signal pathway expression, while NBPF1 was silenced, showing contrary results. Our data 
suggested that NBPF1 overexpression may be a suppressor for cervical cancer via affecting cell invasion and 
apoptosis through regulating PI3K/mTOR signaling pathway. NBPF1 may be a potential therapeutic target for 
cervical cancer treatment.
KW  - Cervical cancer; Neuroblastoma breakpoint family member 1 (NBPF1); Cell apoptosis;  Cell invasion; PI3K/mTOR pathway

DO  - 10.3727/096504015X14410238486766