TY  - EJOU
AU  - Zhen, Yan 
AU  - Li, Dongming 
AU  - Li, Wen 
AU  - Yao, Weimin 
AU  - Wu, Aibing 
AU  - Huang, Jing 
AU  - Gu, Hongli 
AU  - Huang, Yujie 
AU  - Wang, Yajun 
AU  - Wu, Jun 
AU  - Chen, Min 
AU  - Wu, Dong 
AU  - Lyu, Quanchao 
AU  - Fang, Weiyi 
AU  - Wu, Bin 

TI  - Reduced PDCD4 Expression Promotes Cell Growth Through PI3K/Akt  Signaling in Non-Small Cell Lung Cancer
T2  - Oncology Research

PY  - 2015
VL  - 23
IS  - 1-2
SN  - 1555-3906

AB  - It is largely recognized that PDCD4 is frequently lost in tumors of various origins, including lung cancer, and 
its loss contributes to tumor progression. However, its role and molecular mechanism remain largely unexplored in non-small cell lung cancer (NSCLC). In this study, downregulated PDCD4 mRNA expression was 
found in NSCLC tissues compared to their corresponding paracarcinoma tissues and distal paracarcinoma tissues. Induced expression of PDCD4 inhibited cell growth and proliferation and cell cycle transition in vitro. 
Conversely, knocking down PDCD4 expression promoted cell growth and proliferation. Mechanistically, 
PDCD4 inactivated PI3K/Akt signaling and its downstream cell cycle factors CCND1 and CDK4 to regulate 
cell growth in NSCLC. Additionally, PI3K-specific inhibitor Ly294002 suppressed the expression of pPI3K 
(Tyr458), pAkt (Ser473), CCND1, and CDK4 in PC9-shPDCD4 and A549-shPDCD4 cells. Furthermore, Aktspecific inhibitor MK2206 inhibited the expression of pAkt (Ser473), CCND1, and CDK4 in PC9-shPDCD4 
and A549-shPDCD4 cells. Taken together, our study provides evidence that PDCD4 inhibits cell growth 
through PI3K/Akt signaling in NSCLC and may be a potential therapeutic target for NSCLC.
KW  - PDCD4; Non-small cell lung cancer (NSCLC); Cell growth; PI3K/Akt

DO  - 10.3727/096504015X14478843952861