@Article{096504015X14478843952906,
AUTHOR = {Encieh Choghaei, Gholamreza Khamisipour, Mojtaba Falahati, Behrooz Naeimi, Majid Mossahebi-Mohammadi, Rahim Tahmasebi, Mojtaba Hasanpour, Shakib Shamsian, Zahra Sadat Hashemi},
TITLE = {Knockdown of microRNA-29a Changes the Expression of Heat Shock  Proteins in Breast Carcinoma MCF-7 Cells},
JOURNAL = {Oncology Research},
VOLUME = {23},
YEAR = {2015},
NUMBER = {1-2},
PAGES = {69--78},
URL = {http://www.techscience.com/or/v23n1-2/57572},
ISSN = {1555-3906},
ABSTRACT = {Breast cancer is the most commonly occurring cancer among women. MicroRNAs as noncoding small RNA 
molecules play pivotal roles in cancer-related biological processes. Increased levels of microRNA-29a in the 
serum of breast cancer patients have been reported. Since heat shock proteins (HSPs) play important roles in cell 
events, the quantitative fluctuations in their cellular levels could be deemed as key indicators of how the exerted 
treatment alters cell behavior. In this regard, using an antisense small RNA, we attempted to investigate the effects 
of miR-29a knockdown on the expression of HSPs genes in the MCF-7 breast cancer cell line. MCF-7 cells were 
cultured in high-glucose Dulbecco’s modified Eagle’s medium with 10% FBS. Studied cells were subdivided 
into five groups: treated with scramble, anti-miR-29a, anti-miR-29a+Taxol, Taxol, and control. Taxol was added 
24 h post-anti-miR transfection and RNA extraction, and cDNA synthesis was done 48 h later. The changes in 
expression of HSP27, HSP40, HSP60, HSP70, and HSP90 were evaluated by real-time PCR. Our results revealed 
that inhibitors of microRNA-29a promote apoptosis through upregulation of HSP60 level and downregulation 
of HSP27, HSP40, HSP70, and HSP90 levels and could be contemplated as a compelling alternative for Taxol 
employment with similar effects and/or to sensitize cancer cells to chemotherapy with fewer side effects.},
DOI = {10.3727/096504015X14478843952906}
}