@Article{096504015X14500513118029,
AUTHOR = {Qun Zhao, Bi-Bo Tan, Yong Li, Li-Qiao Fan, Pei-Gang Yang, Yuan Tian},
TITLE = {Enhancement of Drug Sensitivity by Knockdown of HIF-1α in Gastric Carcinoma Cells},
JOURNAL = {Oncology Research},
VOLUME = {23},
YEAR = {2015},
NUMBER = {3},
PAGES = {129--136},
URL = {http://www.techscience.com/or/v23n3/57561},
ISSN = {1555-3906},
ABSTRACT = {In this study, the effects of hypoxia-inducible factor-1α (HIF-1α) on gastric carcinoma (GC) drug resistance 
through apoptosis-related genes are investigated. First, HIF-1α-specific siRNA was synthetized and transfected 
into drug-resistant GC cell line OCUM-2MD3/L-OHP. Then MTT assay was applied to test the inhibition rate 
of GC cells by 5-fluorouracil (5-FU) and oxaliplatin (L-OHP). After that, flow cytometry (FCM) was applied 
to measure apoptosis rate. qPCR and Western blot assay were employed to detect HIF-1α and apoptosis-related 
genes. Results showed that HIF-1α in OCUM-2MD3/L-OHP cells was higher than that in OCUM-2MD3 
and gastric epithelial cells. After HIF-1α-siRNA transfection, inhibition rates of 5-FU and L-OHP to tumor 
cells increased significantly. FCM results showed that apoptosis rate of OCUM-2MD3/L-OHP cells increased 
significantly. After HIF-1α-siRNA transfection, survivin and Bcl-2 decreased, whereas Bax, caspase 3, and 
caspase 8 increased significantly. Results from this study seem to confirm that HIF-1α getting involved in GC 
drug resistance is possibly due to its regulation of some apoptosis-related genes. HIF-1α may be a potential 
target to reverse drug resistance of GC.},
DOI = {10.3727/096504015X14500513118029}
}