@Article{096504016X14519157902645,
AUTHOR = {Zhong Yu, Xu Zai-Chun, Han Wun-Lun, Zhu Yun-Yun},
TITLE = {BMP-7 Attenuates TGF-β1-Induced Fibronectin Secretion and Apoptosis of NRK-52E Cells by the Suppression of miRNA-21},
JOURNAL = {Oncology Research},
VOLUME = {23},
YEAR = {2015},
NUMBER = {4},
PAGES = {147--154},
URL = {http://www.techscience.com/or/v23n4/57551},
ISSN = {1555-3906},
ABSTRACT = {Bone morphogenetic protein-7 (BMP-7) inhibited the pathogenesis of renal injury in response to a variety of 
stimuli. However, little is known about the molecular regulation and mechanism of endogenous BMP-7 and 
its renoprotective functions. This study examined the regulation of BMP-7 and its role in the fibronectin secretion and apoptosis of NRK-52E cells resulting from transforming growth factor-β1 (TGF-β1) in vitro. Results 
showed that TGF-β1 promoted factor-associated suicide (FAS), FAS ligand (FASL), fibronectin (FN), and 
miRNA-21 expression, while it downregulated phospho-Smad1 (pSmad1), pSmad5, and pSmad8 expressions 
in NRK-52E cells. In contrast, BMP-7 alleviated TGF-β1-induced cell apoptosis, inhibited TGF-β1-induced 
higher expression of miRNA-21 and FN, and enhanced TGF-β1-attenuated phosphorylation of Smad1, Smad5, 
and Smad8. Furthermore, a chemical inhibitor of miRNA-21 also negatively affected TGF-β1-induced apoptosis and FN secretion. On the other hand, overexpression of miRNA-21 counteracted the inhibitory effect of 
BMP-7 on TGF-β1-induced FN secretion and apoptosis. However, BMP-7 showed no effects on TGF-β1-induced 
FN secretion and apoptosis following knockdown of miRNA-21. Taken together, these findings demonstrated 
that BMP-7 might inhibit TGF-β1-induced FN secretion and apoptosis by the suppression of miRNA-21 in 
NRK-52E cells.},
DOI = {10.3727/096504016X14519157902645}
}