@Article{096504016X14562725373752,
AUTHOR = {Ming Liu, Dan Wang, Ning Li},
TITLE = {MicroRNA-20b Downregulates HIF-1α and Inhibits the Proliferation and Invasion of Osteosarcoma Cells},
JOURNAL = {Oncology Research},
VOLUME = {23},
YEAR = {2015},
NUMBER = {5},
PAGES = {257--266},
URL = {http://www.techscience.com/or/v23n5/57550},
ISSN = {1555-3906},
ABSTRACT = {Osteosarcoma (OS) is the most common malignant primary bone tumor disease. HIF-1α was predicted to be 
the target gene of microRNA-20b (miR-20b). The present study was designed to illustrate the effect of miR-
20b in regulating osteosarcoma via targeting HIF-1α. In this study, we found that the expression of HIF-1α
was significantly increased, while miR-20b obviously decreased in OS patients and OS cell lines compared 
with healthy controls. Moreover, the luciferase report confirmed the targeting reaction between miR-20b and 
HIF-1α. Additionally, the overexpression of miR-20b suppressed the invasion and growth of both MG63 and 
U2OS cells, and inhibited the expressions of HIF-1α and VEGF pathway proteins, while the inhibition of 
miR-20b led to the reverse results. Furthermore, the overexpression of HIF-1α affected the suppression effect 
of miR-20b in MG63 cells, indicating that miR-20b suppresses the tumor cell process via inhibiting the expression of HIF-1α. Taken together, our results suggest that the upregulation of miR-20b affects the expression of 
HIF-1α, downregulates the VEGF pathway proteins, and suppresses cell invasion and proliferation rate. These 
results provide a potential therapeutic strategy for osteosarcoma.},
DOI = {10.3727/096504016X14562725373752}
}