@Article{096504016X14611963142290,
AUTHOR = {Kang Zhu, Ying He, Cui Xia, Jing Yan, Jin Hou, Demin Kong, Yeye Yang, Guoxi Zheng},
TITLE = {MicroRNA-15a Inhibits Proliferation and Induces Apoptosis in CNE1  Nasopharyngeal Carcinoma Cells},
JOURNAL = {Oncology Research},
VOLUME = {24},
YEAR = {2016},
NUMBER = {3},
PAGES = {145--151},
URL = {http://www.techscience.com/or/v24n3/56960},
ISSN = {1555-3906},
ABSTRACT = {Nasopharyngeal carcinoma (NPC) is a highly metastatic cancer, frequently occurring in Southeast Asia and 
Southern China. Several microRNAs (miRNAs) have been shown to have an inhibitive effect on NPC, while 
the effect of miR-15a on NPC remains unclear. Thus, our study aimed to investigate the potential effect of 
miR-15a on NPC cell proliferation, apoptosis, and possible functional mechanism. Human NPC CNE1 cells 
were transfected with miR-15a mimics, miR-15a inhibitors, or a control. Afterward, cell viability and apoptosis 
were assayed by using CCK-8, BrdU assay, and flow cytometry. Moreover, Western blot was used to detect 
the expression changes of proliferation and apoptosis of related proteins. As a result, miR-15a overexpression 
significantly reduced cell proliferation (<i>p</i><0.01 or <i>p</i><0.001) and induced cell apoptosis (<i>p</i><0.001), while miR-
15a suppression got the opposite result for cell proliferation and apoptosis. In addition, miR-15a overexpression upregulated the protein levels of p27, GSK-3β, Bax, procaspase 3, and active caspase 3, whereas miR-15a 
suppression downregulated these proteins. The protein level of p21 was not significantly regulated by miR-15a 
overexpression or suppression. These results indicated that miR-15a played a role for inhibition of proliferation 
and induction of apoptosis in CNE1 cells.},
DOI = {10.3727/096504016X14611963142290}
}