@Article{096504016X14652175055765,
AUTHOR = {Xiaobin Hou, Tinghui Li, Zhipeng Ren, Yang Liu},
TITLE = {Novel BRCA2-Interacting Protein, LIMD1, Is Essential for the  Centrosome Localization of BRCA2 in Esophageal Cancer Cell},
JOURNAL = {Oncology Research},
VOLUME = {24},
YEAR = {2016},
NUMBER = {4},
PAGES = {247--253},
URL = {http://www.techscience.com/or/v24n4/56972},
ISSN = {1555-3906},
ABSTRACT = {Mutation of breast cancer 2, early onset (BRCA2) has been identified as a vital risk factor for esophageal cancer 
(EC). To date, several proteins have been reported as BRCA2-interacting proteins and are associated with multiple biological processes. This study's aim was to identify a novel interactive protein of BRCA2 and to explore 
its functional roles in EC. A yeast two-hybrid screening was performed to identify a novel BRCA2-interacting 
protein. Glutathione-S-transferase (GST) pull-down analysis was performed to find out how the binding domain 
of BRCA2 interacts with LIM domains containing 1 (LIMD1). The interaction between LIMD1 and BRCA2 
at the endogenous level was confirmed by using coimmunoprecipitation and immunobloting. Furthermore, 
two different sequences of short hairpin RNAs (shRNAs) against LIMD1 were transfected into the human EC 
cell line ECA109. Afterward, the effects of LIMD1 suppression on the centrosome localization of BRCA2 
and cell division were analyzed using an immunofluorescence microscope. Results showed that LIMD1 was a 
novel BRCA2-interacting protein, and LIMD1 interacted with the conserved region of BRCA2 (amino acids 
2,750–3,094) in vitro. Importantly, after interfering with the protein expression of LIMD1 in ECA109 cells, the 
centrosome localization of BRCA2 was significantly abolished and abnormal cell division was significantly 
increased. These results suggested that LIMD1 is a novel BRCA2-interacting protein and is involved in the 
centrosome localization of BRCA2 and suppression of LIMD1, causing abnormal cell division in EC cells.},
DOI = {10.3727/096504016X14652175055765}
}