@Article{096504016X14685034103356,
AUTHOR = {Tao Liang, Xiao-Yong Hu, Yong-Hui Li, Bin-Qiang Tian, Zuo-Wei Li, Qiang Fu},
TITLE = {MicroRNA-21 Regulates the Proliferation, Differentiation, and Apoptosis of  Human Renal Cell Carcinoma Cells by the mTOR-STAT3 Signaling Pathway},
JOURNAL = {Oncology Research},
VOLUME = {24},
YEAR = {2016},
NUMBER = {5},
PAGES = {371--380},
URL = {http://www.techscience.com/or/v24n5/56986},
ISSN = {1555-3906},
ABSTRACT = {MicroRNA-21 (miRNA-21), a kind of short, noncoding RNAs, functioned as a tumor marker and was upregulated in renal cell carcinoma (RCC). However, the underlying mechanisms of miRNA-21 in RCC were uncertain. Therefore, the effects and mechanisms of miRNA-21 on the proliferation, differentiation, and apoptosis 
of cultured human RCC cells were further investigated in this study. After slicing miRNA-21 in RCC cells, 
the viability, mRNA expression of C/EBPα and PPARγ, caspase 3 activity, and protein expression of mTOR, 
STAT3, and pSTAT3 were determined. It was found that knockdown of miRNA-21 downregulated the optical density (OD) value of cells, inhibited mRNA expression of PPARγ and C/EBPα, and enhanced activity 
of caspase 3. Furthermore, protein expression of pSTAT3 was also decreased in the absence of miRNA-21. 
Notably, miRNA-21-changed proliferation, differentiation, and apoptosis of human RCC cells were partially 
regulated following the block of the mTOR-STAT3 signaling pathway by the mTOR inhibitor (XL388). It was 
indicated that miRNA-21 promoted proliferation and differentiation and decreased apoptosis of human RCC 
cells through the activation of the mTOR-STAT3 signaling pathway.},
DOI = {10.3727/096504016X14685034103356}
}