@Article{096504016X14666990347518,
AUTHOR = {Jing Li, Weixing Qu, Yazhou Jiang, Yi Sun, Yongyi Cheng, Tiejun Zou, Shuangkuan Du},
TITLE = {miR-489 Suppresses Proliferation and Invasion of Human Bladder Cancer Cells},
JOURNAL = {Oncology Research},
VOLUME = {24},
YEAR = {2016},
NUMBER = {6},
PAGES = {391--398},
URL = {http://www.techscience.com/or/v24n6/56988},
ISSN = {1555-3906},
ABSTRACT = {   MicroRNAs (miRNAs) have been shown to be involved in bladder cancer progression. miR-489 (also known 
as miR-489-3p) was recently reported to be a tumor suppressor in several cancers. However, its exact role 
and mechanism in the progression of bladder cancer are largely unknown. In this study, we explore the role 
of miR-489 in the proliferation and invasion of human bladder cancer cells. The miR-489 expression levels 
were detected in bladder cancer and normal adjacent tissues, as well as in human normal bladder epithelial 
cells and bladder cancer cell lines. The results showed that miR-489 was sharply reduced in bladder cancer 
tissues and cell lines. Then the miR-489 mimic or oligo anta-miR-489 was transfected into T24 and UMUC3 
bladder cancer cell lines. The results showed that the miR-489 mimic greatly increased the miR-489 level and 
significantly decreased the proliferation and invasion of T24 and UMUC3 cells. In contrast, the anta-miR-
489 had a completely opposite effect on miR-489 expression, cell proliferation, and cell invasion. Moreover, 
bioinformatics and luciferase reporter gene assays confirmed that miR-489 targeted the mRNA 3'-untranslated 
region (3'-UTR) region of Jagged1 (JAG1), a Notch ligand. In conclusion, miR-489 suppressed proliferation 
and invasion of human bladder cancer cells.},
DOI = {10.3727/096504016X14666990347518}
}