@Article{096504016X14685034103473,
AUTHOR = {Fu-qiang Zhu, Li Zeng, Na Tang, Ya-ping Tang, Bo-ping Zhou, Fang-fang Li, Wei-gang Wu, Xiao-bing Zeng, Shu-song Peng},
TITLE = {MicroRNA-155 Downregulation Promotes Cell Cycle Arrest and Apoptosis  in Diffuse Large B-Cell Lymphoma},
JOURNAL = {Oncology Research},
VOLUME = {24},
YEAR = {2016},
NUMBER = {6},
PAGES = {415--427},
URL = {http://www.techscience.com/or/v24n6/56991},
ISSN = {1555-3906},
ABSTRACT = {Diffuse large B-cell lymphoma (DLBCL) is the most common non-Hodgkin’s lymphoma in the adult population, and treatment of DLBCL is still unfavorable. Therefore, there is an urgent requirement to investigate the 
molecular mechanisms underlying DLBCL tumorigenesis. To study the potential function of microRNA-155 
(miR-155) involved in the regulation of lymphoma, we monitored lymphoma cell behavior including proliferation, cell cycle, and apoptosis using CCK-8 and flow cytometry analysis. Real-time PCR was used to detect the 
expression levels of miR-155 in 118 lymphoma patients’ tissues, and Western blot was also used to analyze the 
expression level of proteins correlated with cell cycle and apoptosis in lymphoma cells. miR-155 expression 
levels were higher in lymphoma tissues compared with adjacent tissues. Downregulation of miR-155 inhibited 
lymphoma cell progress by arresting cell cycle in the G<sub>0</sub>/G<sub>1</sub> phase and promoting apoptosis. Cell cycle-correlated 
proteins (cyclin B1, cyclin D1, and CDK4) were inhibited by downregulation of miR-155. Apoptosis-correlated 
proteins level (Bax/Bcl-2 and caspase 3 activity) were increased by downregulation of miR-155. In addition, 
a significant inverse correlation between the level of miR-155 and transforming growth factor-β receptor 2 
(TGFBR2) was observed, which has been demonstrated to be a novel tumor suppressor gene. A further in vivo 
tumor formation study in nude mice indicated that downregulation of miR-155 in lymphoma cells delayed the 
progress of tumor formation. These findings indicate that miR-155 may serve as a useful potential target for 
the treatment of lymphoma.},
DOI = {10.3727/096504016X14685034103473}
}