@Article{096504016X14685034103518,
AUTHOR = {Changqing Pan, Dan Wang, Yao Zhang, Wenliang Yu},
TITLE = {MicroRNA-1284 Inhibits Cell Viability and Induces Apoptosis of Ovarian  Cancer Cell Line OVCAR3},
JOURNAL = {Oncology Research},
VOLUME = {24},
YEAR = {2016},
NUMBER = {6},
PAGES = {429--435},
URL = {http://www.techscience.com/or/v24n6/56992},
ISSN = {1555-3906},
ABSTRACT = {Ovarian cancer is a malignancy with high mortality among women. Multiple reports show that microRNAs 
(miRs) act as regulators in ovarian cancer inhibition, while the role of miR-1284 in ovarian cancer is still 
unknown. This study aimed to investigate the effects of miR-1284 on ovarian cancer cells. Human ovarian cancer cell line OVCAR3 was cultured and transfected with miR-1284 mimics, inhibitors, or control. Viability and 
apoptosis of transfected cells were then determined by MTT assay, BrdU assay, and flow cytometry. Expression 
changes of p27, p21, and PI3K/Akt pathway-related proteins were measured by Western blot. Results showed 
that miR-1284 overexpression suppressed cell viability while increasing the apoptosis in OVCAR3 cells. 
Moreover, the expression level of p27 was upregulated by miR-1284 overexpression. Furthermore, miR-1284 
overexpression and Akt inhibitor GSK690693 downregulated the levels of p-Akt and Bcl-2 while upregulating the levels of Bax and caspase 3. However, miR-1284 suppression attenuated the regulatory effects of 
GSK690693 on these proteins. In conclusion, miR-1284 could inhibit cell viability via regulating the expression of p27 and induce apoptosis via regulating the PI3K/Akt pathway in OVCAR3 cells.},
DOI = {10.3727/096504016X14685034103518}
}