TY  - EJOU
AU  - Miao, Yi 
AU  - Lu, Meng 
AU  - Yan, Qin 
AU  - Li, Shuangdi 
AU  - Feng, Youji 

TI  - Inhibition of Proliferation, Migration, and Invasion by Knockdown of  Pyruvate Kinase-M2 (PKM2) in Ovarian Cancer SKOV3 and OVCAR3 Cells
T2  - Oncology Research

PY  - 2016
VL  - 24
IS  - 6
SN  - 1555-3906

AB  - Pyruvate kinase (PK) is a key enzyme in the process of glycolysis, catalyzing phosphoenolpyruvate (PEP) 
into pyruvate. Currently, PK isozyme type M2 (PKM2), one subtype of PK, has been proposed as a new 
tumor marker with high expression in various tumor tissues. Here we aimed to explore the effects of siRNAPKM2 on ovarian carcinoma (OC) cell lines SKOV3 and OVCAR3, in which PKM2 was notably expressed. 
PKM2 gene interference lentivirus vectors were built by miRNA transfection assay. siRNA-PKM2-transfected 
SKOV3 and OVCAR3 cells were evaluated for cell proliferation, cell cycle distribution, cell apoptosis, cell 
migration, and invasion in this study. In addition, the expression levels of several tumor-related genes were 
measured using real-time PCR and Western blot. Results showed that siRNA-PKM2 markedly inhibited cell 
proliferation, induced apoptosis, and caused cell cycle arrest at the G<sub>0</sub>/G<sub>1</sub> phase. Cell migration and invasion 
were significantly suppressed by siRNA-PKM2. Furthermore, the tumor-related genes caspase 7, Bad, and 
E-cadherin were upregulated, while MMP2, HIF1a, VEGF, and MMP9 were depressed by siRNA-PKM2. The 
function of siRNA-PKM2 on the biological behavior of OC cells indicated that PKM2 may also be a target for 
treatment of OC.
KW  - Pyruvate kinase isozyme type M2 (PKM2); Ovarian carcinoma (OC); Cell proliferation;  Metastasis; Signaling pathway

DO  - 10.3727/096504016X14685034103671