@Article{096504016X14685034103752,
AUTHOR = {Jian Zhang, ZhenFeng Shi, JinXing Huang, XiaoGuang Zou},
TITLE = {CSTB Downregulation Promotes Cell Proliferation and Migration and  Suppresses Apoptosis in Gastric Cancer SGC-7901 Cell Line},
JOURNAL = {Oncology Research},
VOLUME = {24},
YEAR = {2016},
NUMBER = {6},
PAGES = {487--494},
URL = {http://www.techscience.com/or/v24n6/57006},
ISSN = {1555-3906},
ABSTRACT = {This study aimed to investigate the pivotal role of cystatin B (CSTB) in the development of gastric cancer and 
to explore its possible regulatory mechanism. Human gastric cancer SGC-7901 cells as a model in vitro were 
transfected with plasmid PCDNA3.1-CSTB and siRNA-CSTB using Lipofectamine 2000. Quantitative realtime PCR (qRT-PCR) and Western blotting were performed to determine the relative expression of CSTB and 
PI3K/Akt/mTOR pathway-related protein. Moreover, MTT assay, Transwell assay, and flow cytometry were 
used to assess cell proliferation, migration, and apoptosis, respectively. The results showed that CSTB was 
significantly downregulated in SGC-7901 cells compared with gastric epithelial cells. CSTB was successfully 
overexpressed and suppressed after cells were transfected with pc-CSTB and si-CSTB, respectively. Moreover, 
cell viability and migration were significantly decreased after being transfected with pc-CSTB when compared 
with the control group, while being obviously increased after transfection with si-CSTB. However, cell apoptosis was significantly induced after being transfected with pc-CSTB, while being obviously suppressed after 
transfection with si-CSTB. Besides, the expression levels of p-PI3K, p-Akt, and p-mTOR proteins were all 
significantly decreased in the pc-CSTB transfection group when compared with the control group, while being 
increased in the si-CSTB transfection group. Our findings suggest that CSTB downregulation may promote the 
development of gastric cancer by affecting cell proliferation and migration, and the PI3K/Akt/mTOR signaling pathway was activated in this process. CSTB may serve as a potential therapeutic target for gastric cancer.},
DOI = {10.3727/096504016X14685034103752}
}