@Article{096504016X14719078133249,
AUTHOR = {Hailin Pang, Ningqiang Ma, Mi Jiao, Weiwei Shen, Bo Xin, Tongfei Wang, Feng Zhang, Lili Liu, Helong Zhang},
TITLE = {The Biological Effects of Dickkopf1 on Small Cell Lung Cancer Cells  and Bone Metastasis},
JOURNAL = {Oncology Research},
VOLUME = {25},
YEAR = {2017},
NUMBER = {1},
PAGES = {35--42},
URL = {http://www.techscience.com/or/v25n1/56780},
ISSN = {1555-3906},
ABSTRACT = {The bone is among the most common sites of metastasis in patients with lung cancer. Over 30%–40% of lung 
cancers can develop bone metastasis, and no effective therapeutic methods exist in clinic cases. Wnt/β-catenin 
signaling and Dickkopf1 (DKK1) play important roles in the progression of lung cancer, which preferentially 
metastasizes to the skeleton. However, the role of DKK1 in osteotropism of small cell lung cancer (SCLC)
remains to be elucidated. This study aimed to define the role of DKK1 in SCLC bone metastasis and investigate the underlying mechanisms. Our results demonstrated that the expression level of DKK1 was dramatically 
higher in bone metastatic SCLC cells (SBC-5 cell line) compared with that in cells without bone metastatic 
ability (SBC-3 cell line). Therefore, we hypothesized that DKK1 was involved in the bone metastasis of SCLC. 
We then suppressed the DKK1 expression in SBC-5 cells by RNAi and found that downregulation of DKK1 
can inhibit cell proliferation, colony formation, cell migration, and invasion, but increase the apoptosis rate. 
Downregulation of DKK1 did not affect the cell cycle progression of SBC-5 cells in vitro. In vivo, downregulated DKK1 in SBC-5 cells resulted in attenuated bone metastasis. These results indicated that DKK1 may be 
an important regulator in bone metastases of SCLC, and targeting DKK1 may be an effective method to prevent 
and treat skeleton metastases in SCLC cases.},
DOI = {10.3727/096504016X14719078133249}
}