@Article{096504016X14719078133401,
AUTHOR = {Minglei Zhang, Dapeng Wang, Tongtong Zhu, Ruofeng Yin},
TITLE = {miR-214-5p Targets ROCK1 and Suppresses Proliferation and Invasion  of Human Osteosarcoma Cells},
JOURNAL = {Oncology Research},
VOLUME = {25},
YEAR = {2017},
NUMBER = {1},
PAGES = {75--81},
URL = {http://www.techscience.com/or/v25n1/56784},
ISSN = {1555-3906},
ABSTRACT = {MicroRNAs (miRNAs) are small conserved RNAs regulating specific target genes in posttranscriptional 
levels. They have been involved in multiple processes of tumor progression, including cell proliferation. 
miR-214-5p (also miR-214*) is a newly identified miRNA, and its functions are largely unknown. In this 
study, we explore the role of miR-214-5p in the proliferation and invasion of human osteosarcoma (OS) 
cells. The results showed that miR-214-5p was sharply reduced in OS tissues and cell lines, compared 
with normal tissues and cell lines. In addition, the miR-214-5p mimic greatly increased the miR-214-5p 
level and significantly decreased the proliferation and invasion of HOS and G293 OS cells. In contrast, the 
miR-214-5p inhibitor had a completely opposite effect on the miR-214-5p level, cell proliferation, and cell 
invasion. Moreover, bioinformatics and luciferase reporter gene assays confirmed that miR-1908 targeted 
the mRNA 3'-UTR region of ROCK1, a characterized tumor promoter in OS. In conclusion, miR-214-5p 
was identified as a new tumor suppressor, which directly targeted ROCK1 and suppressed proliferation of 
human OS cells.},
DOI = {10.3727/096504016X14719078133401}
}