@Article{096504016X14732772150262,
AUTHOR = {Zhiying Su, Hua Yang, Min Zhao,‡ Yanlong Wang, Guoyi Deng, Ruixin Chen},
TITLE = {MicroRNA-92a Promotes Cell Proliferation in Cervical Cancer  via Inhibiting p21 Expression and Promoting Cell Cycle Progression},
JOURNAL = {Oncology Research},
VOLUME = {25},
YEAR = {2017},
NUMBER = {1},
PAGES = {137--145},
URL = {http://www.techscience.com/or/v25n1/56791},
ISSN = {1555-3906},
ABSTRACT = {MicroRNA-92a (miR-92a) generally plays a promoting role in human cancers, but the underlying mechanism 
in cervical cancer remains unclear. Here we studied the expression and clinical significance of miR-92a in 
cervical cancer, as well as the regulatory mechanism in the proliferation of cervical cancer cells. Our data indicated that miR-92a was significantly upregulated in cervical cancer tissues compared to their matched adjacent 
nontumor tissues (ANTs), and the increased miR-92a levels were significantly associated with a higher grade, 
lymph node metastasis, and advanced clinical stage in cervical cancer. In vitro study revealed that inhibition 
of miR-92a led to a significant reduction in the proliferation of HeLa cells via induction of cell cycle arrest 
at the G<sub>1</sub> stage. In contrast, overexpression of miR-92a markedly promoted the proliferation of HeLa cells by 
promoting cell cycle progression. Further investigation revealed that miR-92a has a negative effect on protein levels, but not the mRNA levels, of p21 in HeLa cells, suggesting that p21 is a direct target of miR-92a. 
Overexpression of p21 eliminated the promoting effects of miR-92a on the proliferation and cell cycle progression of HeLa cells. However, knockdown of p21 reversed the suppressive effects of miR-92a downregulation 
on HeLa cell proliferation and cell cycle progression. Moreover, p21 was significantly downregulated in cervical cancer tissues compared to ANTs, suggesting that the increased expression of miR-92a may contribute 
to the decreased expression of p21, which further promotes cervical cancer growth. In conclusion, our study 
demonstrates that miR-92a promotes the proliferation of cervical cancer cells via inhibiting p21 expression and 
promoting cell cycle progression, highlighting the clinical significance of miR-92a in cervical cancer.},
DOI = {10.3727/096504016X14732772150262}
}