@Article{096504016X14732514133203,
AUTHOR = {Ke Wang, Yu Ren, Yang Liu, Jian Zhang, Jian-jun He},
TITLE = {miR-4262 Promotes Proliferation and Invasion of Human Breast  Cancer Cells Through Directly Targeting KLF6 and KLF15},
JOURNAL = {Oncology Research},
VOLUME = {25},
YEAR = {2017},
NUMBER = {2},
PAGES = {277--283},
URL = {http://www.techscience.com/or/v25n2/56807},
ISSN = {1555-3906},
ABSTRACT = {miRNAs have been shown to be involved in breast cancer growth and progression. miR-4262 is a potential 
tumor promoter in human cancers. In this study, we first investigated the role of miR-4262 in the proliferation 
and invasion of human breast cancer cells. Our results showed that, compared with the adjacent tissues and 
MCF-10A normal breast epithelial cells, miR-4262 was markedly increased in the breast cancer tissues and 
five cell lines, including MDA-MB-231, MDA-MB-468, MDA-MB-435, SKBR3, and MCF-7. Then the miR-
4262 mimic or oligo anta-miR-4262 was transfected into MDA-MB-231 and MCF-7 breast cancer cell lines. 
The results showed that the miR-4262 mimic greatly increased the miR-4262 level and the proliferation and 
invasion of MDA-MB-231 and MCF-7 cells. In contrast, the anta-miR-4262 had a completely opposite effect 
on miR-4262 expression, cell proliferation, and cell invasion in MDA-MB-231 and MCF-7 cells. Moreover, 
bioinformatics and luciferase reporter gene assays confirmed that miR-4262 targeted the mRNA 3'-UTR region 
of KLF6 and KLF15, two characterized tumor suppressor genes. miR-4262 suppressed protein levels of KLF6 
and KLF15 in MDA-MB-231 cells, and the suppression could be rescued by the transfection of pcDNA-KLF6 
and -KLF15. In conclusion, miR-4262 positively regulates proliferation and invasion of human breast cancer 
cells via suppression of KLF6 and KLF15.},
DOI = {10.3727/096504016X14732514133203}
}