@Article{096504016X14741511303522,
AUTHOR = {Gulijiahan Aierken, Ayinuer Seyiti, Mayinuer Alifu, Gulina Kuerban},
TITLE = {Knockdown of Tripartite-59 (TRIM59) Inhibits Cellular Proliferation  and Migration in Human Cervical Cancer Cells},
JOURNAL = {Oncology Research},
VOLUME = {25},
YEAR = {2017},
NUMBER = {3},
PAGES = {381--388},
URL = {http://www.techscience.com/or/v25n3/56817},
ISSN = {1555-3906},
ABSTRACT = {The tripartite motif (TRIM) family of proteins is a class of highly conservative proteins that have been implicated in multiple processes. TRIM59, one member of the TRIM family, has now received recognition as a key 
regulator in the development and progression of human diseases. However, its role in human tumorigenesis has 
remained largely unknown. In this study, the effects of TRIM59 expression on cell proliferation and migration 
were investigated in human cervical cancer cells. The expression of TRIM59 in clinical cervical cancer tissues and cervical cancer cells was initially determined by RT-PCR and Western blot. Specific shRNA against 
TRIM59 was then employed to knock down the expression of TRIM59 in cervical cancer lines HeLa and SiHa. 
The effects of TRIM59 knockdown on cell proliferation was assessed by MTT assay and colony formation 
assay. Transwell assay was conducted to reveal cell migration and invasion abilities before and after TRIM59 
knockdown. Our results showed that the expression of TRIM59 was significantly elevated in cervical cancers. 
Knockdown of TRIM59 significantly inhibited cell proliferation and colony formation as well as cell migration and invasion abilities in cervical cancer HeLa and SiHa cells. Cell cycle progression analysis showed that 
TRIM59-depleted cells preferred to accumulate in the S phase. These data suggest that TRIM59 is a potential 
target that promotes the progression of cervical cancer.},
DOI = {10.3727/096504016X14741511303522}
}