TY  - EJOU
AU  - Zhou, Yong 
AU  - Yang, Chuandong 
AU  - Wang, Kunpeng 
AU  - Liu, Xuefeng 
AU  - Liu, Quan 

TI  - MicroRNA-33b Inhibits the Proliferation and Migration of Osteosarcoma  Cells via Targeting Hypoxia-Inducible Factor-1α
T2  - Oncology Research

PY  - 2017
VL  - 25
IS  - 3
SN  - 1555-3906

AB  - Recently, microRNA (miR)-33b has been demonstrated to act as a tumor suppressor in osteosarcoma. However, 
the regulatory mechanism of miR-33b in osteosarcoma cell proliferation and migration remains largely 
unknown. In this study, real-time PCR showed that miR-33b was significantly downregulated in osteosarcoma 
tissues compared to their matched adjacent nontumor tissues. Its expression was also decreased in several common osteosarcoma cell lines, including Saos-2, MG63, U2OS, and SW1353, when compared to normal osteoblast cell line hFOB. Overexpression of miR-33b suppressed U2OS cell proliferation and migration. HIF-1α
was further identified as a target of miR-33b, and its protein levels were reduced after overexpression of miR-
33b in U2OS cells. Moreover, overexpression of HIF-1α significantly reversed the suppressive effect of miR-
33b on U2OS cell proliferation and migration. In addition, HIF-1α was found to be significantly upregulated in 
osteosarcoma tissues compared to adjacent nontumor tissues, and their expression levels were inversely correlated to the miR-33b levels in osteosarcoma tissues. According to these findings, miR-33b plays a suppressive 
role in the regulation of osteosarcoma cell proliferation and migration via directly targeting HIF-1α. Therefore, 
we suggest that the miR-33b/HIF-1α axis may become a promising therapeutic target for osteosarcoma.
KW  - Osteosarcoma; MicroRNA; Proliferation; Migration; Hypoxia-inducible factor-1α

DO  - 10.3727/096504016X14743337535446