TY  - EJOU
AU  - Kouzegaran, Samaneh 
AU  - Shahraki, Kourosh 
AU  - Makateb, Ali 
AU  - Shahri, Farkhondeh 
AU  - Hatami, Negin 
AU  - Behnod, Vahid 
AU  - Tanha, Amir Saber 

TI  - Prognostic Investigations of Expression Level of Two Genes FasL and Ki-67  as Independent Prognostic Markers of Human Retinoblastoma
T2  - Oncology Research

PY  - 2017
VL  - 25
IS  - 4
SN  - 1555-3906

AB  - In this study, expression of FasL and Ki-67 messenger RNA (FasL and Ki-67 mRNA) in human retinoblastoma (HRB) was examined by the immunohistochemistry method and quantitative real-time PCR. Positive 
expression of Ki-67 in tumor cells was detected in 16 of 30 patients (53.33%), and only 9 (30%) of the tissues 
from patients with retinoblastoma showed positive staining for FasL. Our results revealed that FasL expression was significantly higher in tumor tissue with invasion compared with the noninvasion form (<i>p</i> = 0.033). 
Ki-67 expression was markedly increased in tumor tissues with invasion compared with the noninvasion group 
(<i>p</i> = 0.04), but no significant correlation was found between FasL expression and differentiation (<i>p</i>>0.05). In 
addition, Ki-67 expression was strongly linked to differentiation (<i>p</i><0.002). Expression of these FasL was correlated with shorter overall survival of patients, but its expression was not significantly associated with overall 
survival (<i>p</i> = 0.15). The impact of Ki-67 expression on survival in patients was also evaluated. Ki-67 expression 
level was not found to be significantly associated with shorter survival (Kaplan–Meier; <i>p</i> = 0.09). Univariate 
analysis revealed that massive choroidal invasion was correlated with poor prognosis. Taken together, the data 
suggest that massive choroidal invasion is also an important indicator of poor prognosis for HRB.
KW  - Retinoblastoma (RB); Ki-67 and FasL; Analysis; Expression; Molecular

DO  - 10.3727/096504016X14721217330657