TY  - EJOU
AU  - Azizmohammadi, Susan 
AU  - Azizmohammadi, Sima 
AU  - Safari, Aghdas 
AU  - Kaghazian, Maria 
AU  - Sadrkhanlo, Mina 
AU  - Behnod, Vahid 
AU  - Seifoleslami, Mehri 

TI  - High-Level Expression of RIPK4 and EZH2 Contributes  to Lymph Node Metastasis and Predicts Favorable  Prognosis in Patients With Cervical Cancer
T2  - Oncology Research

PY  - 2017
VL  - 25
IS  - 4
SN  - 1555-3906

AB  - The investigation of specific genes will establish more useful biomarkers for accurate detection and management of gynecological cancers, especially patients with cervical cancer (CCP). The aim of this study was 
to evaluate the expression level of RIPK4 and EZH2 messenger RNA (RIPK4 and EZH2 mRNA) in CCP. 
Expression of RIPK4 and EZH2 in the tissues was determined by immunohistochemistry and qRT-PCR methods. 
Correlations of RIPK4 and EZH2 mRNA with clinical and pathological parameters were analyzed using the 
Fisher’s exact test. The mRNA level of RIPK4 was significantly upregulated in tumor tissues compared with 
matched adjacent normal tissues (4.10±0.89 vs. 1.5± 0.82; <i>p</i> = 0.021). EZH2 mRNA was increased in cancer tissues compared to normal tissues (3.54±0.71 vs. 1.2± 0.65; <i>p</i> = 0.003). High expression of RIPK4 was 
observed in 25 patients (64.1%), whereas weak expression was seen in 14 cases (35.9%). Furthermore, the 
expression of RIPK4 was overexpressed in matched adjacent normal tissues (<i>p</i> = 0.004). FIGO stage and lymph 
node metastasis were significantly linked to a higher expression of RIPK4 (<i>p</i><0.05). Overexpression of EZH2 
was found in 30 patients (76.9%) and was associated with FIGO stage, histological type, and lymph node 
metastasis (<i>p</i><0.05). In conclusion, our data suggest that RIPK4/EZH2 markers might be used as potential 
predictors of prognosis in cervical cancer.
KW  - Cervical cancer; Marker; Patient; Pathology; Immunohistochemistry (IHC)

DO  - 10.3727/096504016X14749735594687