@Article{096504016X14768374457986,
AUTHOR = {Chunsheng Li, Jingrong Dong, Zhenqi Han, Kai Zhang},
TITLE = {MicroRNA-219-5p Represses the Proliferation, Migration, and Invasion of  Gastric Cancer Cells by Targeting the LRH-1/Wnt/β-Catenin Signaling Pathway},
JOURNAL = {Oncology Research},
VOLUME = {25},
YEAR = {2017},
NUMBER = {4},
PAGES = {617--627},
URL = {http://www.techscience.com/or/v25n4/56843},
ISSN = {1555-3906},
ABSTRACT = {MicroRNAs (miRNAs) are reportedly involved in gastric cancer development and progression. In particular, 
miR-219-5p has been reported to be a tumor-associated miRNA in human cancer. However, the role of miR-
219-5p in gastric cancer remains unclear. In this study, we investigated for the first time the potential role and 
underlying mechanism of miR-219-5p in the proliferation, migration, and invasion of human gastric cancer 
cells. miR-219-5p was found to be markedly decreased in gastric cancer tissues and cell lines compared with 
adjacent tissues and normal gastric epithelial cells. miR-219-5p mimics or anti-miR-219-5p was transfected 
into gastric cancer cell lines to overexpress or suppress miR-219-5p expression, respectively. Results showed 
that miR-219-5p overexpression significantly decreased the proliferation, migration, and invasion of gastric 
cancer cells. Conversely, miR-219-5p suppression demonstrated a completely opposite effect. Bioinformatics 
and luciferase reporter assays indicated that miR-219-5p targeted the 3'-untranslated region of the liver receptor homolog-1 (LRH-1), a well-characterized oncogene. Furthermore, miR-219-5p inhibited the mRNA and 
protein levels of LRH-1. LRH-1 mRNA expression was inversely correlated with miR-219-5p expression 
in gastric cancer tissues. miR-219-5p overexpression significantly decreased the Wnt/β-catenin signaling 
pathway in gastric cancer cells. Additionally, LRH-1 restoration can markedly reverse miR-219-5p-mediated 
tumor suppressive effects. Our study suggests that miR-219-5p regulated the proliferation, migration, and 
invasion of human gastric cancer cells by suppressing LRH-1. miR-219-5p may be a potential target for 
gastric cancer therapy.},
DOI = {10.3727/096504016X14768374457986}
}