@Article{096504016X14772342320617,
AUTHOR = {Zhiling Zhang, Jiawei Wang, Runfang Gao, Xuan Yang, Yafen Zhang, Jie Li, Jing Zhang, Xingjuan Zhao, Chunfang Xi, Xiaoting Lu},
TITLE = {Downregulation of MicroRNA-449 Promotes Migration and Invasion  of Breast Cancer Cells by Targeting Tumor Protein D52 (TPD52)},
JOURNAL = {Oncology Research},
VOLUME = {25},
YEAR = {2017},
NUMBER = {5},
PAGES = {753--761},
URL = {http://www.techscience.com/or/v25n5/56857},
ISSN = {1555-3906},
ABSTRACT = {Our study aimed to investigate whether microRNA-449 (miR-449) plays a key role in regulating the migration 
and invasion of breast cancer cells via targeting tumor protein D52 (TPD52). The results of the qRT-PCR and 
Western blotting showed that, in comparison with normal breast tissues and cells, miR-449 was significantly 
downregulated in breast cancer tissues and cells, while TPD52 was markedly upregulated. After transfection 
with an miR-449 inhibitor, suppression of miR-449 significantly promoted cell migration and invasion. Also, 
when miR-449 was overexpressed by transfection with miR-449 mimics, E-cadherin expression significantly 
increased, and the expression of N-cadherin and vimentin were markedly decreased, whereas the opposite 
effects were obtained when miR-449 was suppressed by transfection with an miR-449 inhibitor. TPD52 was 
also confirmed as the direct target of miR-449 via luciferase reporter analysis. Knockdown of TPD52 significantly alleviated the effects of miR-449 overexpression on cell migration and invasion, as well as the 
expression of E-cadherin, N-cadherin, and vimentin. Our results indicate that downregulation of miR-449 
may promote the migration and invasion of breast cancer cells by targeting TPD52. miR-449 may serve as a 
potential target in the therapy of breast cancer.},
DOI = {10.3727/096504016X14772342320617}
}