@Article{096504016X14783677992682,
AUTHOR = {Kewei Ren, Zhen Li, Yahua Li, Wenzhe Zhang, Xinwei Han},
TITLE = {Long Noncoding RNA Taurine-Upregulated Gene 1 Promotes Cell Proliferation  and Invasion in Gastric Cancer via Negatively Modulating miRNA-145-5p},
JOURNAL = {Oncology Research},
VOLUME = {25},
YEAR = {2017},
NUMBER = {5},
PAGES = {789--798},
URL = {http://www.techscience.com/or/v25n5/56861},
ISSN = {1555-3906},
ABSTRACT = {Long noncoding RNA (lncRNA) taurine-upregulated gene 1 (TUG1) is involved in the development and 
carcinogenesis of various tumors, suggesting the diagnostic potential of TUG1 in these cancers. However, 
the exact role of TUG1 and its underlying mechanism in gastric cancer (GC) remain unknown. In this study, 
the expression of TUG1 and miR-145-5p in GC cell lines and nonmalignant gastric epithelial cell lines was 
detected by qRT-PCR. BGC-823 and SGC-7901 cells were transfected with si-TUG1, pcDNA 3.1-TUG1, 
miR-145-5p mimics, or matched controls. The biological function of TUG1 and miR-145-5p in GC cell 
proliferation and invasion in vitro and tumor growth in vivo was investigated by MTT assay, Transwell invasion assay, and tumor xenograft experiments. The regulating relationship between TUG1 and miR-145-5 
was confirmed by luciferase reporter assay. The results showed that TUG1 was significantly overexpressed 
and miR-145-5p was dramatically downregulated in GC cell lines. TUG1 knockdown strikingly inhibited 
cell proliferation and invasion in vitro and markedly suppressed tumor growth in vivo. Furthermore, TUG1 
could directly bind to miR-145-5p and repress miR-145-5p expression. TUG1 overexpression significantly 
relieved the inhibition on GC cell proliferation and invasion in vitro and tumor growth in vivo, mediated by 
miR-145-5p overexpression. In conclusion, TUG1 promotes cell proliferation and invasion in GC via negatively modulating miRNA-145-5p, which undoubtedly contributes to understanding the mechanism of GC 
occurrence and development.},
DOI = {10.3727/096504016X14783677992682}
}