@Article{096504016X14783701102564,
AUTHOR = {Jiakai Han, Wei Gao, Dongyue Su, Yang Liu},
TITLE = {Silencing of A-Kinase Anchor Protein 4 (AKAP4)  Inhibits Proliferation and Progression of Thyroid Cancer},
JOURNAL = {Oncology Research},
VOLUME = {25},
YEAR = {2017},
NUMBER = {6},
PAGES = {873--878},
URL = {http://www.techscience.com/or/v25n6/56869},
ISSN = {1555-3906},
ABSTRACT = {A-kinase anchor protein 4 (AKAP4), a member of the A-kinase anchor family of proteins, plays a role in tumor 
development and progression. However, its expression pattern and function in human thyroid cancer remain 
obscure. Here we examined AKAP4 expression in thyroid cancer cell lines as well as the effects of AKAP4 
on the proliferation and metastasis of thyroid cancer cells. We also explored the molecular mechanism by 
which AKAP4 mediates the metastatic potential of thyroid cancer cells. Our results revealed that the transcript 
and protein levels of AKAP4 were significantly upregulated in thyroid cancer cell lines. In vitro experiments 
showed that knockdown of AKAP4 significantly inhibited the proliferation, migration, invasion, and epithelial–
mesenchymal transition (EMT) process in thyroid cancer cells. Additionally, knockdown of AKAP4 greatly 
decreased the protein expression of Shh as well as Smo, Ptc, and Gli-1 in ACT-1 cells. Finally, the in vivo 
nude mice model confirmed that knockdown of AKAP4 attenuated tumor growth. In conclusion, our findings 
demonstrated that knockdown of AKAP4 inhibited proliferation and metastasis, likely through suppressing 
the Shh signaling pathway, in thyroid cancer cells. Thus, AKAP4 may act as a potential therapeutic target for 
human thyroid cancer.},
DOI = {10.3727/096504016X14783701102564}
}