@Article{096504016X14791732531006,
AUTHOR = {Yu Zhou, Yong Peng, Min Liu, Yugang Jiang},
TITLE = {MicroRNA-181b Inhibits Cellular Proliferation and Invasion of Glioma Cells  via Targeting Sal-Like Protein 4},
JOURNAL = {Oncology Research},
VOLUME = {25},
YEAR = {2017},
NUMBER = {6},
PAGES = {947--957},
URL = {http://www.techscience.com/or/v25n6/56877},
ISSN = {1555-3906},
ABSTRACT = {MicroRNAs (miRs), a class of noncoding RNAs that are 18–25 nucleotides in length, are able to suppress 
gene expression by targeting complementary regions of mRNAs and inhibiting protein translation. Recently, 
miR-181b was found to play a suppressive role in glioma, but the regulatory mechanism of miR-181b in the 
malignant phenotypes of glioma cells remains largely unclear. In this study, we found that miR-181b was significantly downregulated in glioma tissues when compared with normal brain tissues, and decreased miR-181b 
levels were significantly associated with high-grade pathology and a poor prognosis for patients with glioma. 
Moreover, miR-181b was downregulated in glioma cell lines (U87, SHG44, U373, and U251) compared to 
normal astrocytes. Overexpression of miR-181b significantly decreased the proliferation, migration, and invasion of glioma U251 cells. Sal-like protein 4 (SALL4) was identified as a novel target gene of miR-181b 
in U251 cells. The expression of SALL4 was significantly upregulated in glioma tissues and cell lines, and 
an inverse correlation was observed between the miR-181b and SALL4 expression levels in glioma. Further 
investigation showed that the protein expression of SALL4 was negatively regulated by miR-181b in U251 
cells. Knockdown of SALL4 significantly inhibited the proliferation, migration, and invasion of U251 cells, 
while overexpression of SALL4 effectively reversed the suppressive effects of miR-181b on these malignant 
phenotypes of U251 cells. In conclusion, our study demonstrates that miR-181b has a suppressive effect on the 
malignant phenotypes of glioma cells, at least partly, by directly targeting SALL4. Therefore, the miR-181b/
SALL4 axis may become a potential therapeutic target for glioma.},
DOI = {10.3727/096504016X14791732531006}
}