TY  - EJOU
AU  - Gu, Yueli 
AU  - Si, Jinchun 
AU  - Xiao, Xichun 
AU  - Tian, Ying 
AU  - Yang, Shuo 

TI  - miR-92a Inhibits Proliferation and Induces Apoptosis by Regulating  Methylenetetrahydrofolate Dehydrogenase 2 (MTHFD2)  Expression in Acute Myeloid Leukemia
T2  - Oncology Research

PY  - 2017
VL  - 25
IS  - 7
SN  - 1555-3906

AB  - Aberrant expression of microRNA-92a (miR-92a) has been investigated in various cancers. However, the function and mechanism of miR-92a in acute myeloid leukemia (AML) remain to be elucidated. Our data showed 
that miR-92a was evidently downregulated and methylenetetrahydrofolate dehydrogenase 2 (MTHFD2) was 
remarkably upregulated in AML cell lines HL-60 and THP-1. Dual luciferase reporter assay revealed that 
MTHFD2 was a direct target of miR-92a. Gain- and loss-of-function analysis demonstrated that MTHFD2 
knockdown or miR-92a overexpression notably inhibited proliferation and promoted apoptosis of AML cell 
lines. Restoration of MTHFD2 expression reversed proliferation inhibition and apoptosis induction of AML 
cells triggered by miR-92a. Moreover, an implanted tumor model in mice indicated that miR-92a overexpression dramatically decreased tumor growth and MTHFD2 expression in vivo. Taken together, our results suggest 
that miR-92a inhibits proliferation and induces apoptosis by directly regulating MTHFD2 expression in AML. 
miR-92a may act as a tumor suppressor in AML, providing a promising therapeutic target for AML patients.
KW  - miR-92a; Methylenetetrahydrofolate dehydrogenase 2 (MTHFD2);  Acute myeloid leukemia (AML); Proliferation; Apoptosis

DO  - 10.3727/096504016X14829256525028