@Article{096504016X14831120463349,
AUTHOR = {Zhangjie Jiang, Yida Zhang, Runfu Cao, Li Li, Kezhao Zhong, Qingsheng Chen, Jianjun Xiao},
TITLE = {miR-5195-3p Inhibits Proliferation and Invasion of Human Bladder Cancer  Cells by Directly Targeting Oncogene KLF5},
JOURNAL = {Oncology Research},
VOLUME = {25},
YEAR = {2017},
NUMBER = {7},
PAGES = {1081--1087},
URL = {http://www.techscience.com/or/v25n7/56890},
ISSN = {1555-3906},
ABSTRACT = {miRNAs play a key role in the carcinogenesis of many cancers, including bladder cancer. In the current 
study, the role of miR-5195-3p, a quite recently discovered and poorly studied miRNA, in the proliferation 
and invasion of human bladder cancer cells was investigated. Our data displayed that, compared with healthy 
volunteers (control) and SU-HUC-1 normal human bladder epithelial cells, miR-5195-3p was sharply downregulated in bladder cancer patients and five human bladder cancer cell lines. The oligo miR-5195-3p mimic 
or miR-5195-3p antagomir was subsequently transfected into both T24 and BIU-87 bladder cancer cell 
lines. The miR-5195-3p mimic robustly increased the miR-5195-3p expression level and distinctly reduced 
the proliferation and invasion of T24 and BIU-87 cells. In contrast, the miR-5195-3p antagomir had an 
opposite effect on miR-5195-3p expression, cell proliferation, and invasion. Our data from bioinformatic 
and luciferase reporter gene assays identified that miR-5195-3p targeted the mRNA 3'-UTR of Krüppel-like 
factor 5 (KLF5), which is a proven proto-oncogene in bladder cancer. miR-5195-3p sharply reduced KLF5 
expression and suppressed the expression or activation of its several downstream genes that are kinases 
improving cell survival or promoting cell cycle regulators, including ERK1/2, VEGFA, and cyclin D1. In 
conclusion, miR-5195-3p suppressed proliferation and invasion of human bladder cancer cells via suppression of KLF5.},
DOI = {10.3727/096504016X14831120463349}
}