@Article{096504017X14841698396829,
AUTHOR = {Bin Xu, Hehuan Xia, Junming Cao, Zhihong Wang, Yipeng Yang, Yongsheng Lin},
TITLE = {MicroRNA-21 Inhibits the Apoptosis of Osteosarcoma Cell Line  SAOS-2 via Targeting Caspase 8},
JOURNAL = {Oncology Research},
VOLUME = {25},
YEAR = {2017},
NUMBER = {7},
PAGES = {1161--1168},
URL = {http://www.techscience.com/or/v25n7/56900},
ISSN = {1555-3906},
ABSTRACT = {Currently, multiple microRNAs (miRNAs) have been found to play vital roles in the pathogenesis of osteosarcoma. This study aimed to investigate the role of miR-21 in osteosarcoma. The level of miR-21 in 20 pairs 
of osteosarcoma and corresponding adjacent tissues was monitored by qPCR. Human osteosarcoma cell line 
SAOS-2 was transfected with either miR-21 mimic or miR-21 inhibitor, and then cell viability, survival, 
and apoptosis were measured by MTT, colony formation assay, and flow cytometry. A target of miR-21 
was predicted by the microRNA.org database and verified in vitro by using luciferase reporter, qPCR, and 
Western blot analyses. Finally, cells were cotransfected with siRNA against caspase 8 and miR-21 inhibitor, 
and the apoptotic cell rate was determined again. Results showed that the mRNA level of miR-21 was highly 
expressed in osteosarcoma tissues compared with adjacent tissues. Overexpression of miR-21 improved 
cell viability and survival but suppressed apoptosis. Caspase 8 was a direct target of miR-21, and it was 
negatively regulated by miR-21. Moreover, miR-21 suppression attenuated caspase 8 silencing and induced 
the decrease in apoptosis. In conclusion, overexpression of miR-21 suppressed SAOS-2 cell apoptosis via 
directly targeting caspase 8.},
DOI = {10.3727/096504017X14841698396829}
}