TY  - EJOU
AU  - Wang, Jin 
AU  - Pang, Wenquan 
AU  - Zuo, Zhenbai 
AU  - Zhang, Wenyan 
AU  - He, Weidong 

TI  - MicroRNA-520b Suppresses Proliferation, Migration, and Invasion  of Spinal Osteosarcoma Cells via Downregulation of Frizzled-8
T2  - Oncology Research

PY  - 2017
VL  - 25
IS  - 8
SN  - 1555-3906

AB  - Spinal osteosarcoma (OS) is a malignant tumor that has a poor outcome. MicroRNA-520b (miR-520b) acts as 
a cancer suppressor in various types of cancer. Because of the limited amount of literature on OS, we aimed to 
identify the role of miR-520b in OS. The miR-520b level in clinical spinal OS tissues and adjacent nontumor 
tissues as well as in cell lines was assessed. The effect of miR-520b on cell proliferation, migration, invasion, 
and frizzled-8 (FZD8) degradation were all evaluated. Alterations of key proteins involved in the Wnt/b-catenin 
pathway were assessed by Western blot analysis. In the present study, miR-520b was downregulated in human 
spinal OS tissues and OS cell lines (<i>p</i><0.01 or <i>p</i><0.001). Overexpression of miR-520b inhibited cell proliferation (<i>p</i><0.01 or <i>p</i><0.001), migration (<i>p</i><0.01), and invasion (<i>p</i><0.01). FZD8 expression was negatively 
regulated by infection with a lentivirus vector carrying an miR-520b precursor in dose- and time-dependent 
manners. In OS tissues, miR-520b was inversely correlated with FZD8 expression. FZD8 was upregulated 
in human spinal OS tissues and cell lines. Finally, miR-520b inactivated the Wnt/β-catenin pathway through 
downregulation of FZD8. miR-520b inhibited cell proliferation, migration, and invasion through inactivating 
the Wnt/β-catenin pathway by downregulation of FZD8, providing a novel therapeutic target for spinal OS.
KW  - miR-520b; Spinal osteosarcoma (OS); Frizzled-8; Wnt/β-catenin pathway

DO  - 10.3727/096504017X14873430389189