@Article{096504017X14874323871217,
AUTHOR = {Junfeng Zhang, Kun Xu, Lili Shi, Li Zhang, Zhaohua Zhao, Hao Xu, Fei Liang, Hongbo Li, Yan Zhao, Xi Xu, Yingfang Tian},
TITLE = {Overexpression of MicroRNA-216a Suppresses Proliferation, Migration,  and Invasion of Glioma Cells by Targeting Leucine-Rich  Repeat-Containing G Protein-Coupled Receptor 5},
JOURNAL = {Oncology Research},
VOLUME = {25},
YEAR = {2017},
NUMBER = {8},
PAGES = {1317--1327},
URL = {http://www.techscience.com/or/v25n8/56916},
ISSN = {1555-3906},
ABSTRACT = {Increasing studies have suggested that microRNAs (miRNAs) are involved in the development of gliomas. 
MicroRNA-216a has been reported to be a tumor-associated miRNA in many types of cancer, either as an 
oncogene or as a tumor suppressor. However, little is known about the function of miR-216a in gliomas. The 
present study was designed to explore the potential role of miR-216a in gliomas. We found that miR-216a was 
significantly decreased in glioma tissues and cell lines. Overexpression of miR-216a significantly suppressed 
the proliferation, migration, and invasion of glioma cells. Leucine-rich repeat-containing G protein-coupled 
receptor 5 (LGR5) was identified as a target gene of miR-216a in glioma cells by bioinformatics analysis, 
dual-luciferase reporter assay, real-time quantitative polymerase chain reaction, and Western blot analysis. 
Moreover, miR-216a overexpression inhibited the Wnt/β-catenin signaling pathway. The restoration of LGR5 
expression markedly reversed the antitumor effect of miR-216a in glioma cells. Taken together, these findings 
suggest a tumor suppressor role for miR-216a in gliomas, which inhibits glioma cell proliferation, migration, 
and invasion by targeting LGR5. Our study suggests that miR-216a may serve as a potential therapeutic target 
for future glioma treatment.},
DOI = {10.3727/096504017X14874323871217}
}