TY  - EJOU
AU  - Wu, Xiaoyu 
AU  - Zhou, Jin 
AU  - Wu, Zhenfeng 
AU  - Chen, Che 
AU  - Liu, Jiayun 
AU  - Wu, Guannan 
AU  - Zhai, Jing 
AU  - Liu, Fukun 
AU  - Li, Gang 

TI  - miR-101-3p Suppresses HOX Transcript Antisense RNA (HOTAIR)-Induced  Proliferation and Invasion Through Directly Targeting SRF  in Gastric Carcinoma Cells
T2  - Oncology Research

PY  - 2017
VL  - 25
IS  - 8
SN  - 1555-3906

AB  - miR-101-3p has been identified as a tumor suppressor in several cancers, but its exact role in gastric adenocarcinoma is still largely unknown. In this study, we found that, compared with the RGM-1 human normal 
gastric epithelial cells, miR-101-3p was significantly downregulated in all six human gastric adenocarcinoma 
cell lines, including BGC-823, MNK-45, MGC-803, SGC-7901, AGS, and HGC-27. Overexpression of miR-
101-3p suppressed both the proliferation and invasion of AGS gastric adenocarcinoma cells, and knockdown 
of miR-101-3p displayed the opposite effect. In addition, miR-101-3p could directly target and suppress 
the expression of the serum response factor (SRF) gene, which is a transcription factor of HOTAIR, a wellcharacterized tumor promoter lncRNA. miR-101-3p negatively regulated SRF-mediated transcription of HOTAIR. 
Moreover, silencing of either SRF or HOTAIR could counteract the promotion of gastric adenocarcinoma cell 
proliferation and invasion by miR-101-3p inhibition. Our findings indicate that miR-101-3p suppresses HOTAIRinduced proliferation and invasion through directly targeting SRF in gastric carcinoma cells.
KW  - miR-101-3p; Gastric carcinoma; Proliferation and invasion; Serum response factor (SRF);  HOX transcript antisense RNA (HOTAIR)

DO  - 10.3727/096504017X14879366402279