@Article{096504016X14826089198805,
AUTHOR = {Shi Ying, Huang Jianjun, Yi Xue, Yu Shuwei, Zhang Liyuan, Wang Jie, Cheng Lixian},
TITLE = {MicroRNA-133b Inhibits Cell Proliferation and Invasion  in Osteosarcoma by Targeting Sirt1},
JOURNAL = {Oncology Research},
VOLUME = {25},
YEAR = {2017},
NUMBER = {9},
PAGES = {1421--1430},
URL = {http://www.techscience.com/or/v25n9/56927},
ISSN = {1555-3906},
ABSTRACT = {MicroRNAs are a class of small noncoding RNAs that function as critical gene regulators through targeting 
mRNAs for translational repression or degradation. In this study, we showed that the miR-133b expression 
level was decreased while the Sirt1 mRNA expression level was increased in osteosarcoma tissue and cell lines. 
A low expression of miR-133b was significantly associated with tumor size, distant metastasis, and advanced 
clinical stage. In addition, osteosarcoma patients with a low miR-133b expression showed a worse prognosis 
when compared to those with a high level of miR-133b expression. Thus, we identified Sirt1 as a novel direct 
target of miR-133b. Overexpression of miR-133b suppressed Sirt1 expression and attenuated cell proliferation 
and invasion. Forced expression of Sirt1 could partly rescue the inhibitory effect of miR-133b in osteosarcoma 
cells. Our finding also suggested that the inhibitory effects of the miR-133b/Sirt1 axis on osteosarcoma progression were involved in the Wnt/β-catenin pathway. Taken together, these findings will shed light on the role 
and mechanism of miR-133b in regulating osteosarcoma cell growth via the miR-133b/Sirt1 axis, and miR-
133b may serve as a potential therapeutic target in osteosarcoma in the future.},
DOI = {10.3727/096504016X14826089198805}
}