@Article{096504017X14886494526344,
AUTHOR = {De-Zhong Zhang, Bing-He Chen, Lan-Fang Zhang, Ming-Kun Cheng, Xiang-Jie Fang, Xin-Jun Wu},
TITLE = {Basic Transcription Factor 3 Is Required for Proliferation and  Epithelial–Mesenchymal Transition via Regulation of  FOXM1 and JAK2/STAT3 Signaling in Gastric Cancer},
JOURNAL = {Oncology Research},
VOLUME = {25},
YEAR = {2017},
NUMBER = {9},
PAGES = {1453--1462},
URL = {http://www.techscience.com/or/v25n9/56930},
ISSN = {1555-3906},
ABSTRACT = {Gastric cancer (GC) is the most common epithelial malignancy worldwide. Basic transcription factor 3 (BTF3) 
plays a crucial role in the regulation of various biological processes. We designed experiments to investigate the 
molecular mechanism underlying the role of BTF3 in GC cell proliferation and metastasis. We confirmed that 
BTF3 expression was decreased in GC tissues and several GC cell lines. Lentivirus-mediated downregulation 
of BTF3 reduced cell proliferation, induced S and G<sub>2</sub>/M cell cycle arrest, and increased apoptosis. Knockdown 
of BTF3 significantly reduced the expression of Forkhead box M1 (FOXM1). Upregulation of FOXM1 significantly inhibited the decrease in cell proliferation due to BTF3 silencing, S and G2/M cell cycle arrest, and 
increase in apoptosis. Knockdown of BTF3 decreased Ki-67 and PCNA expression, whereas it increased p27 
expression, which was inhibited by upregulation of FOXM1. Knockdown of BTF3 significantly decreased 
the ability to invade and migrate. Moreover, knockdown of BTF3 increased E-cadherin expression, whereas 
it decreased N-cadherin and ZEB2 expression, indicating a decrease in epithelial–mesenchymal transition 
(EMT). Phosphorylation of Janus kinase 2/signal transducer and activator of transcription 3 (JAK2/STAT3) 
was significantly inhibited by knockdown of BTF3. IL-6-stimulated phosphorylation of STAT3 and JAK2 
markedly suppressed inhibition of EMT due to BTF3 silencing. Silencing of BTF3 decreased tumor volume 
and weight and reduced peritoneal nodules in implanted tumors. Our findings provide a novel understanding 
of the mechanism of GC and highlight the important role of BTF3/FOXM1 in tumor growth and BTF3/JAK2/
STAT3 in EMT and metastasis.},
DOI = {10.3727/096504017X14886494526344}
}