@Article{096504017X14886689179993,
AUTHOR = {Nan Qin, Gui-Feng Tong, Li-Wei Sun, Xiao-Lin Xu},
TITLE = {Long Noncoding RNA MEG3 Suppresses Glioma Cell Proliferation, Migration,  and Invasion by Acting as a Competing Endogenous RNA of miR-19a},
JOURNAL = {Oncology Research},
VOLUME = {25},
YEAR = {2017},
NUMBER = {9},
PAGES = {1471--1478},
URL = {http://www.techscience.com/or/v25n9/56932},
ISSN = {1555-3906},
ABSTRACT = {Glioma, with varying malignancy grades and histological subtypes, is the most common primary brain tumor 
in adults. Long noncoding RNAs (lncRNAs) are non-protein-coding transcripts and have been proven to play 
an important role in tumorigenesis. Our study aims to elucidate the combined effect of lncRNA maternally 
expressed gene 3 (MEG3) and microRNA-19a (miR-19a) in human glioma U87 and U251 cell lines. Real-time 
PCR revealed that MEG3 was downregulated and miR-19a was upregulated in malignant glioma tissues and 
cell lines. Bioinformatics analyses (TargetScan, miRanda, and starBase V2.0) showed that phosphatase and 
tensin homolog (PTEN) is a target of miR-19a with complementary binding sites in the 3'-UTR. As expected, 
luciferase results verified the putative target site and also revealed the complementary binding between miR-
19a and MEG3. miR-19a represses the expression of PTEN and promotes glioma cell proliferation, migration, 
and invasion. However, MEG3 could directly bind to miR-19a and effectively act as a competing endogenous 
RNA (ceRNA) for miR-19a to suppress tumorigenesis. Our study is the first to demonstrate that lncRNA 
MEG3 suppresses glioma cell proliferation, migration, and invasion by acting as a ceRNA of miR-19a, which 
provides a novel insight about the pathogenesis of glioma.},
DOI = {10.3727/096504017X14886689179993}
}