@Article{096504017X14928634401204,
AUTHOR = {Ce Ji, Ying Zhao, You-Wei Kou, Hua Shao, Lin Guo, Chen-Hui Bao, Ben-Chun Jiang, Xin-Ying Chen, Jing-Wei Dai, Yu-Xin Tong, Ren Yang, Wei Sun, Qiang Wang},
TITLE = {Cathepsin F Knockdown Induces Proliferation and Inhibits Apoptosis  in Gastric Cancer Cells},
JOURNAL = {Oncology Research},
VOLUME = {26},
YEAR = {2018},
NUMBER = {1},
PAGES = {83--93},
URL = {http://www.techscience.com/or/v26n1/56620},
ISSN = {1555-3906},
ABSTRACT = {Gastric cancer (GC) is one of the most common cancers in the world. The cathepsin F (CTSF) gene has recently 
been found to participate in the progression of several types of cancer. However, the clinical characteristics 
and function of CTSF in GC as well as its molecular mechanisms are not clear. Six GC cell lines and 44 paired 
adjacent noncancerous and GC tissue samples were used to assess CTSF expression by quantitative polymerase 
chain reaction (qPCR). We used lentivirus-mediated small hairpin RNA (Lenti-shRNA) against CTSF to knock 
down the expression of CTSF in GC cells. Western blot and qPCR were used to analyze the mRNA and related 
protein expression. The biological phenotypes of gastric cells were examined by cell proliferation and apoptosis assays. Microarray-based mRNA expression profile screening was also performed to evaluate the potential 
molecular pathways in which CTSF may be involved. The CTSF mRNA level was associated with tumor 
differentiation, depth of tumor invasion, and lymph node metastasis. Downregulation of CTSF expression efficiently inhibited apoptosis and promoted the proliferation of GC cells. Moreover, a total of 1,117 upregulated 
mRNAs and 1,143 downregulated mRNAs were identified as differentially expressed genes (DEGs). Further 
analysis identified the involvement of these mRNAs in cancer-related pathways and various other biological 
processes. Nine DEGs in cancer-related pathways and three downstream genes in the apoptosis pathway were 
validated by Western blot, which was mainly in agreement with the microarray data. To our knowledge, this 
is the first report investigating the effect of CTSF on the growth and apoptosis in GC cells and its clinical significance. The CTSF gene may function as a tumor suppressor in GC and may be a potential therapeutic target 
in the treatment of GC.},
DOI = {10.3727/096504017X14928634401204}
}