@Article{096504017X15031557924150,
AUTHOR = {Yinfang Wu, Jun Zhang, Yu Zheng, Cheng Ma, Xing-E Liu, Xiaodong Sun},
TITLE = {miR-216a-3p Inhibits the Proliferation, Migration, and Invasion  of Human Gastric Cancer Cells via Targeting RUNX1  and Activating the NF-κB Signaling Pathway},
JOURNAL = {Oncology Research},
VOLUME = {26},
YEAR = {2018},
NUMBER = {1},
PAGES = {157--171},
URL = {http://www.techscience.com/or/v26n1/56627},
ISSN = {1555-3906},
ABSTRACT = {This work aims to elucidate the effects and the potential underlying mechanisms of microRNA-216a-3p (miR-
216a-3p) on the proliferation, migration, and invasion of gastric cancer (GC) cells. In this study, we revealed 
that the expression of miR-216a-3p was significantly elevated in GC tissues and cell lines. The different expression level of miR-216a-3p was firmly correlated with clinicopathological characteristics of GC patients. We 
next demonstrated that upregulation of miR-216a-3p could dramatically promote the ability of proliferation, 
migration, and invasion of GC cells using a series of experiments, whereas downregulation essentially inhibited 
these properties. Additionally, through bioinformatics analysis and biological approaches, we confirmed that 
runt-related transcription factor 1 (RUNX1) was a direct target of miR-216a-3p, and overexpression of RUNX1 
could reverse the potential effect of miR-216a-3p on GC cells. Furthermore, mechanistic investigation using 
Western blot analysis showed that downregulation of RUNX1 by miR-216a-3p could stimulate the activation 
of NF-kB signaling pathway. In summary, this work proved that miR-216a-3p can promote GC cell proliferation, migration, and invasion via targeting RUNX1 and activating the NF-kB signaling pathway. Therefore, 
miR-216a-3p/RUNX1 could be a possible molecular target for innovative therapeutic agents against GC.},
DOI = {10.3727/096504017X15031557924150}
}