TY  - EJOU
AU  - Li, Heming 
AU  - Li, Junfeng 
AU  - Yang, Taisheng 
AU  - Lin, Shuwen 
AU  - Li, Heng 

TI  - MicroRNA-433 Represses Proliferation and Invasion of Colon Cancer Cells  by Targeting Homeobox A1
T2  - Oncology Research

PY  - 2018
VL  - 26
IS  - 2
SN  - 1555-3906

AB  - The aberrant expression of miR-433 has been validated in some types of cancers. However, the expression profile and the biological function of miR-433 on colon cancer are still elusive. This study was designed to investigate the function of miR-433 on the proliferation and invasion of colon cancer cells. We detected the expression 
of miR-433 in colon cancer tissues, adjacent normal tissues, and cell lines. CCK8 and Transwell assays were 
performed to explore the impact of miR-433 on colon cancer cell proliferation and invasion. The luciferase 
reporter assay was applied to identify the direct target of miR-433. The results demonstrated that miR-433 was 
downregulated in colon cancer tissues and cell lines when compared with the control. Overexpression of miR-
433 significantly suppressed the ability of colon cancer cell proliferation and invasion, whereas knockdown 
of miR-433 remarkably enhanced cell proliferation and invasion. Homeobox A1 (HOXA1) was identified as a 
target of miR-433, and it mediated the functions of miR-433 on colon cancer cells. To conclude, we revealed 
that miR-433 was downregulated in colon cancer, and it inhibited colon cancer cell proliferation and invasion 
by directly targeting HOXA1.
KW  - miR-433; Colon cancer; Proliferation; Invasion; Homeobox A1 (HOXA1)

DO  - 10.3727/096504017X15067856789781