@Article{096504017X14920318811712,
AUTHOR = {Jing Xiao, Guang Li, Jingyu Zhou, Shalong Wang, Dongcai Liu, Guoshun Shu, Jianping Zhou, Feng Ren},
TITLE = {MicroRNA-520b Functions as a Tumor Suppressor in Colorectal Cancer by  Inhibiting Defective in Cullin Neddylation 1 Domain Containing 1 (DCUN1D1)},
JOURNAL = {Oncology Research},
VOLUME = {26},
YEAR = {2018},
NUMBER = {4},
PAGES = {593--604},
URL = {http://www.techscience.com/or/v26n4/56672},
ISSN = {1555-3906},
ABSTRACT = {MicroRNAs (miRs), a class of small noncoding RNAs, are important regulators for gene expression through 
directly binding to the 3'-untranslated region (3'-UTR) of their target mRNA. Recently, downregulation of 
miR-520b has been observed in several common human cancers. However, the exact role of miR-520b in 
colorectal cancer (CRC) has not previously been studied. In this study, our data showed that miR-520b was 
significantly downregulated in CRC and cell lines when compared with adjacent normal tissues and a normal 
intestinal epithelial cell line. Low expression of miR-520b was notably associated with the malignant progress 
and a shorter survival time for CRC patients. Restoration of miR-520b inhibited cell proliferation, migration, 
invasion, and epithelial–mesenchymal transition (EMT) in CRC cells. Defective in cullin neddylation 1 domain 
containing 1 (DCUN1D1) was then identified as a novel target gene of miR-520b in CRC cells. The expression of DCUN1D1 was significantly increased in CRC, with a negative correlation to miR-520b expression 
in CRC tissues. Moreover, a high expression of DCUN1D1 was significantly associated with the malignant 
progress and a poor prognosis for CRC patients. Furthermore, overexpression of DCUN1D1 rescued the miR-
520b-mediated malignant phenotypes and EMT in CRC cells. The data demonstrate that miR-520b functions 
as a tumor suppressor in CRC through targeting DCUN1D1, suggesting that miR-520b may become a potential 
therapeutic target for the treatment of CRC.},
DOI = {10.3727/096504017X14920318811712}
}