@Article{096504017X14953948675395,
AUTHOR = {Pengwei Lu, Yuanting Gu, Lin Li, Fang Wang, Xue Yang, Yunqing Yang},
TITLE = {Long Noncoding RNA CAMTA1 Promotes Proliferation and Mobility of the  Human Breast Cancer Cell Line MDA-MB-231 via Targeting miR-20b},
JOURNAL = {Oncology Research},
VOLUME = {26},
YEAR = {2018},
NUMBER = {4},
PAGES = {625--635},
URL = {http://www.techscience.com/or/v26n4/56675},
ISSN = {1555-3906},
ABSTRACT = {Breast cancer is a serious threat to women’s physical and psychological health. Long noncoding RNA CAMTA1 
(lncCAMTA1) was believed to be related with tumor progression, but its role in breast cancer is not clear. 
The human breast cancer cell line MDA-MB-231 was used to investigate the effect of lncCAMTA1 on cell 
viability, migration/invasion, and apoptosis. The expression of lncCAMTA1, miR-20b, and VEGF in MDAMB-231 were measured after corresponding transfections. Binding effects between lncCAMTA1 and miR-20b, 
miR-20b, and VEGF 3'-UTR were measured. The effects of miR-20b and VEGF on breast cancer cells were 
also assessed after transfections. The phosphorylation levels of the MAPK/ERK and JAK/STAT3 pathways 
were determined to assess the effect of VEGF. The results showed that lncCAMTA1 expression promoted cell 
viability and migration/invasion, while knockdown of lncCAMTA1 promoted cell apoptosis via binding with 
miR-20b. lncCAMTA1 negatively regulated miR-20b expression. VEGF was a target of miR-20b, leading to 
the modification of the phosphorylation levels of MAPK, ERK, JAK, STAT1, and STAT3. Our findings suggested that lncCAMTA1 might promote proliferation and mobility of human breast cancer cells via binding 
with miR-20b. VEGF was a direct target of miR-20b and regulated activation of the MAPK/ERK and JAK/
STAT3 signaling pathways. Therefore lncCAMTA1 has potential as a novel cancer diagnostic marker and as a 
putative novel therapeutic target for breast cancer treatment.},
DOI = {10.3727/096504017X14953948675395}
}