TY  - EJOU
AU  - Lian, Yifan 
AU  - Fan, Weiming 
AU  - Huang, Yanlin 
AU  - Wang, Hongbo 
AU  - Wang, Jialiang 
AU  - Zhou, Liang 
AU  - Wu, Xiaojuan 
AU  - Deng, Meihai 
AU  - Huang, Yuehua 

TI  - Downregulated Trophinin-Associated Protein Plays a Critical Role  in Human Hepatocellular Carcinoma Through Upregulation  of Tumor Cell Growth and Migration
T2  - Oncology Research

PY  - 2018
VL  - 26
IS  - 5
SN  - 1555-3906

AB  - Trophinin-associated protein (TROAP) was a protein first identified to mediate the process of embryo transplantation and later found to be involved in microtubule regulation. However, little is known about the role 
of TROAP in hepatocellular carcinoma (HCC). In the present study, we reported that both TROAP mRNA 
and protein expressions were downregulated in human HCC samples as well as cell lines. A high level of 
TROAP was associated with small tumor size (<i>p</i><0.05), minor tumor nodules (<i>p</i><0.01), and mild vein invasion (<i>p</i><0.05). We further constructed in vitro TROAP depletion and overexpression HCC cell models. TROAP 
depletion significantly enhanced the proliferation and colony formation abilities, whereas TROAP overexpression had an inhibitory effect on the growth of HCC cells. The G<sub>1</sub>/S phase arrest by TROAP overexpression 
correlated with increased cell cycle inhibitors p21 and p27, and declined cell cycle promoting kinase complex 
CDK6/cyclin D1. Depressed TROAP expression enhanced the migration ability, while the opposite influence 
was observed in TROAP-overexpressed HCC cells. Taken together, these results indicate that TROAP suppresses cellular growth and migration in HCC. This discovery will further our understanding of the pathogenic 
mechanisms of human HCC.
KW  - Trophinin-associated protein (TROAP); Hepatocellular carcinoma (HCC); Cell cycle; Tumor growth; Migration

DO  - 10.3727/096504017X15101398724809