TY  - EJOU
AU  - Kong, Qinglong 
AU  - Shu, Nan 
AU  - Li, Jun 
AU  - Xu, Ning 

TI  - miR-641 Functions as a Tumor Suppressor by Targeting MDM2  in Human Lung Cancer
T2  - Oncology Research

PY  - 2018
VL  - 26
IS  - 5
SN  - 1555-3906

AB  - Lung cancer is the leading cause of deaths due to cancer. Studies suggest an important role of microRNAs 
(miRNAs) in a variety of cancers, including lung cancer. In the present study, we evaluated the role of miR-
641 in human lung cancer A549 cells. Quantitative RT-PCR and Western blot were used to measure mRNA 
and protein expression, respectively. Cell viability and cell apoptosis were respectively measured by MTT 
assay and flow cytometry. In addition, luciferase activity assay was used to identify the target of miR-641. 
The expression of miR-641 was downregulated in lung cancer tissues and lung cancer cell lines (<i>p</i><0.05 or 
<i>p</i><0.01). Overexpression of miR-641 significantly inhibited proliferation and induced apoptosis of lung cancer 
cells (<i>p</i>< 0.05, <i>p</i><0.01, or <i>p</i><0.001). MDM2 was identified as a direct target of miR-641. Overexpression of 
miR-641 decreased the expression of MDM2 and increased the expression of p53 in lung cancer cells.
KW  - miR-641; Cell proliferation; Murine double minute 2 (MDM2); Lung cancer;  p53; Apoptosis

DO  - 10.3727/096504017X15021536183490