@Article{096504017X15024946480113,
AUTHOR = {Daofu Jia, Yanping Niu, Dongling Li, Zhaorui Liu},
TITLE = {lncRNA C2dat1 Promotes Cell Proliferation, Migration, and Invasion  by Targeting miR-34a-5p in Osteosarcoma Cells},
JOURNAL = {Oncology Research},
VOLUME = {26},
YEAR = {2018},
NUMBER = {5},
PAGES = {753--764},
URL = {http://www.techscience.com/or/v26n5/56688},
ISSN = {1555-3906},
ABSTRACT = {Osteosarcoma is a highly aggressive malignant bone tumor with poor prognosis. Evidence has suggested that 
lncRNAs are deregulated in multiple cancers. In this study, we investigated the role of the lncRNA C2dat1 on 
the biological functions of osteosarcoma cells. The expressions of C2dat1, miR-34a-5p, and Sirt1 in human 
osteosarcoma cells were altered by transfection with their specific vectors/shRNA or mimic/inhibitor. Cell 
viability, migration, invasion, and apoptosis were assessed posttransfection. The mRNA and protein levels of 
C2dat1, miR-34a-5p, and Sirt1 were detected by qRT-PCR and Western blot. The results showed that C2dat1 
suppression reduced cell viability, invasion, and migration, whereas it increased cell apoptosis in OS-732 cells. 
The expression of miR-34a-5p was downregulated when C2dat1 was overexpressed, whereas it negatively 
regulated Sirt1 expression. miR-34a-5p overexpression inhibited cell viability, migration, and invasion and 
promoted cell apoptosis in osteosarcoma cells by downregulation of Sirt1. Furthermore, miR-34a-5p overexpression deactivated the p38/ERK/AKT and Wnt/b-catenin signaling pathways by inhibition of Sirt1.},
DOI = {10.3727/096504017X15024946480113}
}