TY  - EJOU
AU  - Ma, Chao 
AU  - Han, Jinfeng 
AU  - Dong,  
AU  - Wang, Nanya 

TI  - MicroRNA-152 Suppresses Human Osteosarcoma Cell Proliferation  and Invasion by Targeting E2F Transcription Factor 3
T2  - Oncology Research

PY  - 2018
VL  - 26
IS  - 5
SN  - 1555-3906

AB  - MicroRNA-152 (miR-152) expression has been reported to be downregulated in osteosarcoma (OS). However, 
the role of miR-152 in OS is not well documented. In the present study, we aimed to explore the function and 
underlying mechanism of miR-152 in OS. We found that miR-152 was underexpressed in OS tissues and cell 
lines. Decreased miR-152 was inversely correlated with lymph node metastasis and advanced clinical stage. 
Overexpression of miR-152 significantly inhibited cell proliferation, colony formation, migration, and invasion 
of OS cells. Bioinformatics analyses showed that miR-152 directly targeted E2F transcription factor 3 (E2F3), 
as further confirmed by a dual-luciferase reporter assay. E2F3 expression was upregulated and inversely correlated with miR-152 expression level in human OS tissues. Moreover, the inhibitory effects of miR-152 on 
OS growth and invasion were attenuated by E2F3 overexpression. Taken together, our findings indicated that 
miR-152 reduced OS growth and invasion by targeting E2F3 and provided new evidence of miR-152 as a 
potential therapeutic target for OS.
KW  - Osteosarcoma (OS); miR-152; E2F transcription factor 3 (E2F3); Proliferation; Invasion

DO  - 10.3727/096504017X15021536183535