TY  - EJOU
AU  - Song, Jifu 
AU  - Guan, Zhibin 
AU  - Li, Maojiang 
AU  - Sha,  
AU  - Song, Chao 
AU  - Gao, Zhiwei 
AU  - Zhao, Yongli 

TI  - MicroRNA-154 Inhibits the Growth and Invasion of Gastric Cancer Cells  by Targeting DIXDC1/WNT Signaling
T2  - Oncology Research

PY  - 2018
VL  - 26
IS  - 6
SN  - 1555-3906

AB  - MicroRNAs (miRNAs) have emerged as pivotal regulators of the development and progression of gastric 
cancer. Studies have shown that miR-154 is a novel cancer-associated miRNA involved in various cancers. 
However, the role of miR-154 in gastric cancer remains unknown. Here we aimed to investigate the biological 
function and the potential molecular mechanism of miR-154 in gastric cancer. We found that miR-154 was significantly downregulated in gastric cancer tissues and cell lines. The overexpression of miR-154 significantly 
repressed the growth and invasion of gastric cancer cells. Bioinformatics analysis and Dual-Luciferase Reporter 
Assay data showed that miR-154 directly targeted the 3'-untranslated region of Dishevelled–Axin domain containing 1 (DIXDC1). Real-time quantitative polymerase chain reaction and Western blot analyses showed that 
miR-154 overexpression inhibited DIXDC1 expression. An inverse correlation of miR-154 and DIXDC1 was 
also demonstrated in gastric cancer specimens. Overexpression of miR-154 also significantly suppressed the 
activation of WNT signaling. Moreover, restoration of DIXDC1 expression significantly reversed the inhibitory effect of miR-154 overexpression on the cell proliferation, invasion, and WNT signaling in gastric cancer 
cells. Overall, these results suggest that miR-154 inhibits gastric cancer cell growth and invasion by targeting 
DIXDC1 and could serve as a potential therapeutic target for the treatment of gastric cancer.
KW  - Dishevelled–Axin domain containing 1 (DIXDC1); Gastric cancer; miR-154;  Wingless-related integration site (WNT)

DO  - 10.3727/096504017X15016337254632