@Article{096504017X15024935181289,
AUTHOR = {Cheng Zhang, Jing-Yi Li, Fu-Zhou Tian, Gang Zhao, Hai Hu, Yue-Feng Ma, Yu-Long Yang},
TITLE = {Long Noncoding RNA NEAT1 Promotes Growth and Metastasis  of Cholangiocarcinoma Cells},
JOURNAL = {Oncology Research},
VOLUME = {26},
YEAR = {2018},
NUMBER = {6},
PAGES = {879--888},
URL = {http://www.techscience.com/or/v26n6/56701},
ISSN = {1555-3906},
ABSTRACT = {Long noncoding RNAs (lncRNAs) are known to play important roles in cancers. However, little is known 
about lncRNAs in cholangiocarcinoma (CCA), a cholangiocyte malignancy with poor prognosis. We investigated the role of nuclear paraspeckle assembly transcript 1 (NEAT1) lncRNA in promoting CCA. qRT-PCR 
analysis of patient samples showed that NEAT1 expression was higher in CCA tumors than in matched adjacent nontumor tissue. NEAT1 levels were also higher in CCA cell lines than in a normal biliary epithelium 
cell line (HIBEpic). NEAT1 knockdown in CCA cell lines using shNEAT1 reduced cell proliferation and 
colony formation in CCK-8 and colony formation assays, respectively. CCA cells transfected with shNEAT1 
also exhibited reduced metastasis and invasiveness in Transwell assays. NEAT1 knockdown cells produced 
smaller tumors, demonstrating that NEAT1 promotes tumor growth in vivo. Silencing of NEAT1 increased 
E-cadherin expression in vitro, and E-cadherin expression was inversely correlated with NEAT1 expression 
in CCA tissue samples. RIP and ChIP assays suggest that NEAT1 is recruited to the E-cadherin promoter by 
EZH2 (enhancer of zeste homolog 2), where it represses E-cadherin expression. These findings indicate that 
NEAT1 exerts oncogenic effects in CCA. We postulate that NEAT1 is a potentially useful diagnostic and 
therapeutic target for CCA.},
DOI = {10.3727/096504017X15024935181289}
}