@Article{096504018X15148192843443,
AUTHOR = {Haiyan Jia, Hong Wang, Yanfen Yao, Chunlei Wang, Pibao Li},
TITLE = {miR-136 Inhibits Malignant Progression of Hepatocellular Carcinoma Cells  by Targeting Cyclooxygenase 2},
JOURNAL = {Oncology Research},
VOLUME = {26},
YEAR = {2018},
NUMBER = {6},
PAGES = {967--976},
URL = {http://www.techscience.com/or/v26n6/56710},
ISSN = {1555-3906},
ABSTRACT = {MicroRNAs (miRNAs) play a vital role in regulating tumor progression. Dysregulated miR-136 expression 
was linked to the development of various human cancers. In the present study, we investigated the expression 
and relationship of miR-136 and COX2 in hepatocellular carcinoma (HCC) using relevant experiments, involving CCK-8, Transwell assay, and luciferase reporter assay. We demonstrated that miR-136 expression is obviously decreased in HCC tissues and cells, and negatively correlated with the expression of COX2 mRNA. In 
vitro assay revealed that overexpression of miR-136 significantly changed the expression of proliferation- and 
metastasis-related proteins and inhibited the proliferation, migration, and invasion of HepG2 and Hep3B cells. 
Dual-luciferase reporter assay validated that the 3¢-untranslated region (3¢-UTR) of COX2 is a direct target 
of miR-136. Furthermore, COX2 siRNA partially enhanced the miR-136 overexpression-induced inhibitory 
effects. In conclusion, miR-136 was vital in the regulation of HCC cell proliferation and metastasis by targeting 
COX2. Thus, our findings provided novel evidence that miR-136 might be recommended as a potential target 
for the diagnosis and treatment of HCC patients.},
DOI = {10.3727/096504018X15148192843443}
}